Assessment of Burn Cases Visited in Hospital for Surgical Treatment

Background: Burns can be very destructive, and severely endanger the health and lives of humans. It maybe causes disability and even psychological trauma in individuals. Hence; the present study was undertaken for assessing burn cases visited in hospital for surgical treatment. Materials and methods: A total of 269 burn patients were enrolled in the present study. Complete demographic profile and clinical details of all the patients was obtained. All the patients were segregated into various socio-economic classes on the basis of Kuppuswamy's socioeconomic status scale. Swabs were taken from the burn areas and were inoculated into culture media for assessing the growing microorganisms. All the results were recorded in Microsoft excel sheet. Results: Mean hospital stay was found to be 39.6 days. Conservative treatment without surgical intervention was done in 187 patients while surgical treatment was done in 82 patients. Most common microorganism growing in the burn areas were Pseudomonas aeruginosa, Staphylococcus epidermidis, Methicillin-sensitive staphylococcus aureus, Methicillin-resistant staphylococcus aureus, Enterococcus sp. and Escherichia coli. Conclusion: Burn patients should be initially conservatively managed by adequate resuscitation, early wound debridement and closure.

In vitro propagation of Theileria annulata infected schizonts in different media supplemented with heterologous sera.

Efficacy of medium RPMI-1640 (supplied by Gibco USA, Centron and Hi-media) supplemented with horse, donkey, sheep and goat sera was evaluated for in vitro propagation of Theileria annulata (Hisar) infected bovine mononuclear cells. The results were compared with the growth rate in RPMI-1640 supplemented with foetal bovine serum (Gibco). RPMI-1640 (Gibco) proved to be the best medium for in vitro cultivation of the parasite infected cells. Foetal bovine serum could be easily, safely and reliably substituted with goat and sheep sera in the growth medium. Horse and donkey sera also gave comparable growth of T. annulata infected cells in vitro. Successful use of heterologous sera greatly helped in reducing the cost of in vitro cultivation of T. annulata schizonts. These findings have important implications on mass production of an attenuated cell culture vaccine for the control of bovine tropical theileriosis.

Efficacy and safety of unguided percutaneous fine needle aspiration cytology in peripheral lung lesions.

The efficacy and safety of unguided percutaneous fine needle aspiration cytology in peripheral lung lesions were studied in 42 patients. The diagnostic yield of unguided aspiration was 83.3%. The yield in malignant lesion was 85.7% while in benign lesion it was 78.6%. There was no false positive report. Complications seen were asymptomatic pneumothorax and blood tinged sputum in a few cases following aspiration. The procedure was well accepted by the patients. Unguided FNAC is a simple, quick, economical, safe and reliable procedure for the diagnosis of peripheral lung lesions.